The nocturnal helmet gecko, Tarentola chazaliae, discriminates colors in dim moonlight when humans are color blind. The sensitivity of the helmet gecko eye has been calculated to be 350 times higher than human cone vision at the color vision threshold. The optics and the large cones of the gecko are important reasons why they can use color vision at low light intensities. Using photorefractometry and an adapted laboratory Hartmann–Shack wavefront sensor of high resolution, we also show that the optical system of the helmet gecko has distinct concentric zones of different refractive powers, a so-called multifocal optical system. The intraspecific variation is large but in most of the individuals studied the zones differed by 15 diopters. This is of the same magnitude as needed to focus light of the wavelength range to which gecko photoreceptors are most sensitive. We compare the optical system of the helmet gecko to that of the diurnal day gecko, Phelsuma madagascariensis grandis. The optical system of the day gecko shows no signs of distinct concentric zones and is thereby monofocal.